Evaluation and comparison of the effect of hypothermia and ozone on ischemia–reperfusion injury of skeletal muscle in rats

Tourniquet-induced ischemia–reperfusion, which affects local and distant organs, is very common in orthopedic surgery. Hypothermia is used in traumatic tissue during ischemic period commonly. Ozone (O₃) has been recommended as a novel therapeutic agent in various medical conditions. The objective of the study was to evaluate and compare the effect of hypothermia (H) and O₃ on ischemia–reperfusion injury of skeletal muscle in rats by measuring oxidative parameters and inducible nitric oxide synthase (iNOS) levels.

Eighteen rats (Wistar albino) were separated into five groups randomly (sham, IR, IR + H, IR + O₃, IR + H + O₃; n = 6). The lower right extremity of all rats was subjected to 2 h of ischemia and 22 h of reperfusion clamping the common iliac artery and using the rubber-band technique at the level of the lesser trochanter under general anesthesia. Two hours of …